Reference protein database for DIAMOND ====== DIAMOND is a fast and sensitive sequence aligner for protein and translated DNA searches. - Website: https://github.com/bbuchfink/diamond - Citation: Buchfink B, Reuter K, Drost HG. Sensitive protein alignments at tree-of-life scale using DIAMOND. Nature methods. 2021 Apr;18(4):366-8. Built using DIAMOND v2.0.15. Command: ``` diamond makedb --threads # --in input.faa --db WoLr2 ``` Compatible with DIAMOND v0.9.25 and above (database format version 3). Typical search commands: Search protein sequences: ``` diamond blastp -p # -d WoLr2 -q input.fa -o output.m8 ``` Search whole-metagenome sequencing data: ``` diamond blastx -p # -d WoLr2 -q input.R1.fq.gz input.R2.fq.gz -o output.m8 \ --query-gencode 11 ``` This analysis requires at least 5 GB and up to 20 GB memory.