Mapping file error and warning details.

Notes for interpreting this report:

Some general rules about formatting mapping files (see here for additional details):

General issues with your mapping file (i.e., those that do not pertain to a particular cell) will be listed here, if any:

SampleIDBarcodeSequenceLinkerPrimerSequencecenter_nameexperiment_design_descriptioninstrument_modellibrary_construction_protocolplatformcollection_timestampdaydays_since_experiment_startdna_extractedhost_subject_idlatitudelongitudemonthphysical_specimen_locationphysical_specimen_remainingreported_antibiotic_usagesample_typescientific_nametaxon_idyearDescription
10589.L1S8AGCTGACTAGTCGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina10/28/2008280Yes140.015-105.27110UCSDMIYesYesguthuman gut metagenome4081720081_Fece_10_28_2008
10589.L1S140ATGGCAGCTCTAGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina10/28/2008280Yes240.015-105.27110UCSDMIYesYesguthuman gut metagenome4081720082_Fece_10_28_2008
10589.L1S57ACACACTATGGCGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina1/20/20092084Yes140.015-105.2711UCSDMIYesNoguthuman gut metagenome4081720091_Fece_1_20_2009
10589.L1S208CTGAGATACGCGGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina1/20/20092084Yes240.015-105.2711UCSDMIYesNoguthuman gut metagenome4081720092_Fece_1_20_2009
10589.L1S76ACTACGTGTGGTGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina2/17/200917112Yes140.015-105.2712UCSDMIYesNoguthuman gut metagenome4081720091_Fece_2_17_2009
10589.L1S105AGTGCGATGCGTGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina3/17/200917140Yes140.015-105.2713UCSDMIYesNoguthuman gut metagenome4081720091_Fece_3_17_2009
10589.L1S257CCGACTGAGATGGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina3/17/200917140Yes240.015-105.2713UCSDMIYesNoguthuman gut metagenome4081720092_Fece_3_17_2009
10589.L1S281CCTCTCGTGATCGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina4/14/200914168Yes240.015-105.2714UCSDMIYesNoguthuman gut metagenome4081720092_Fece_4_14_2009
10589.L2S240CATATCGCAGTTGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina10/28/2008280Yes240.015-105.27110UCSDMIYesYesleft palmhuman skin metagenome53965520082_L_Palm_10_28_2008
10589.L2S155ACGATGCGACCAGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina1/20/20092084Yes140.015-105.2711UCSDMIYesNoleft palmhuman skin metagenome53965520091_L_Palm_1_20_2009
10589.L2S309CGTGCATTATCAGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina1/20/20092084Yes240.015-105.2711UCSDMIYesNoleft palmhuman skin metagenome53965520092_L_Palm_1_20_2009
10589.L2S175AGCTATCCACGAGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina2/17/200917112Yes140.015-105.2712UCSDMIYesNoleft palmhuman skin metagenome53965520091_L_Palm_2_17_2009
10589.L2S204ATGCAGCTCAGTGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina3/17/200917140Yes140.015-105.2713UCSDMIYesNoleft palmhuman skin metagenome53965520091_L_Palm_3_17_2009
10589.L2S357CTAACGCAGTCAGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina3/17/200917140Yes240.015-105.2713UCSDMIYesNoleft palmhuman skin metagenome53965520092_L_Palm_3_17_2009
10589.L2S222CACGTGACATGTGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina4/14/200914168Yes140.015-105.2714UCSDMIYesNoleft palmhuman skin metagenome53965520091_L_Palm_4_14_2009
10589.L2S382CTCAATGACTCAGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina4/14/200914168Yes240.015-105.2714UCSDMIYesNoleft palmhuman skin metagenome53965520092_L_Palm_4_14_2009
10589.L3S242ACAGTTGCGCGAGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina10/28/2008280Yes140.015-105.27110UCSDMIYesYesright palmhuman skin metagenome53965520081_R_Palm_10_28_2008
10589.L3S378ATCGATCTGTGGGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina10/28/2008280Yes240.015-105.27110UCSDMIYesYesright palmhuman skin metagenome53965520082_R_Palm_10_28_2008
10589.L3S294CACGACAGGCTAGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina1/20/20092084Yes140.015-105.2711UCSDMIYesNoright palmhuman skin metagenome53965520091_R_Palm_1_20_2009
10589.L4S63CTCGTGGAGTAGGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina1/20/20092084Yes240.015-105.2711UCSDMIYesNoright palmhuman skin metagenome53965520092_R_Palm_1_20_2009
10589.L3S313AGTGTCACGGTGGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina2/17/200917112Yes140.015-105.2712UCSDMIYesNoright palmhuman skin metagenome53965520091_R_Palm_2_17_2009
10589.L3S341CAAGTGAGAGAGGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina3/17/200917140Yes140.015-105.2713UCSDMIYesNoright palmhuman skin metagenome53965520091_R_Palm_3_17_2009
10589.L4S112GCGTTACACACAGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina3/17/200917140Yes240.015-105.2713UCSDMIYesNoright palmhuman skin metagenome53965520092_R_Palm_3_17_2009
10589.L3S360CATCGTATCAACGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina4/14/200914168Yes140.015-105.2714UCSDMIYesNoright palmhuman skin metagenome53965520091_R_Palm_4_14_2009
10589.L4S137GAACTGTATCTCGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina4/14/200914168Yes240.015-105.2714UCSDMIYesNoright palmhuman skin metagenome53965520092_R_Palm_4_14_2009
10589.L5S104CAGTGTCAGGACGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina10/28/2008280Yes140.015-105.27110UCSDMIYesYestonguehuman oral metagenome44742620081_Tong_10_28_2008
10589.L5S240CTGGACTCATAGGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina10/28/2008280Yes240.015-105.27110UCSDMIYesYestonguehuman oral metagenome44742620082_Tong_10_28_2008
10589.L5S155ATCTTAGACTGCGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina1/20/20092084Yes140.015-105.2711UCSDMIYesNotonguehuman oral metagenome44742620091_Tong_1_20_2009
10589.L6S20GAGGCTCATCATGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina1/20/20092084Yes240.015-105.2711UCSDMIYesNotonguehuman oral metagenome44742620092_Tong_1_20_2009
10589.L5S174CAGACATTGCGTGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina2/17/200917112Yes140.015-105.2712UCSDMIYesNotonguehuman oral metagenome44742620091_Tong_2_17_2009
10589.L5S203CGATGCACCAGAGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina3/17/200917140Yes140.015-105.2713UCSDMIYesNotonguehuman oral metagenome44742620091_Tong_3_17_2009
10589.L6S68GATACGTCCTGAGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina3/17/200917140Yes240.015-105.2713UCSDMIYesNotonguehuman oral metagenome44742620092_Tong_3_17_2009
10589.L5S222CTAGAGACTCTTGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina4/14/200914168Yes140.015-105.2714UCSDMIYesNotonguehuman oral metagenome44742620091_Tong_4_14_2009
10589.L6S93GATTAGCACTCTGTGCCAGCMGCCGCGGTAACCMESample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. Illumina HiSeq 2000EMP V4 515f,806rbc protocolIllumina4/14/200914168Yes240.015-105.2714UCSDMIYesNotonguehuman oral metagenome44742620092_Tong_4_14_2009