Tue May 31 09:09:50 2016 Command: sortmerna --ref /home/jona1883/software/American-Gut/data/AG/BLOOM.fasta,/home/jona1883/software/American-Gut/ipynb/primary-processing/ag-May-31-2016/02-filtered/observed-blooms/sortmerna_picked_otus/BLOOM -e 1.0 --aligned /home/jona1883/software/American-Gut/ipynb/primary-processing/ag-May-31-2016/02-filtered/observed-blooms/sortmerna_picked_otus/sortmerna_otus -a 28 --id 0.97 --coverage 0.97 --log --otu_map --reads /tmp/SortMeRNAExactMatchFilteruEPh2G.fasta --de_novo_otu -v --best 1 --fastx Process pid = 29586 Parameters summary: Index: /home/jona1883/software/American-Gut/ipynb/primary-processing/ag-May-31-2016/02-filtered/observed-blooms/sortmerna_picked_otus/BLOOM Seed length = 18 Pass 1 = 18, Pass 2 = 9, Pass 3 = 3 Gumbel lambda = 0.588849 Gumbel K = 0.323663 Minimal SW score based on E-value = 48 Number of seeds = 2 Edges = 4 (as integer) SW match = 2 SW mismatch = -3 SW gap open penalty = 5 SW gap extend penalty = 2 SW ambiguous nucleotide = -3 SQ tags are not output Number of threads = 28 Reads file = /tmp/SortMeRNAExactMatchFilteruEPh2G.fasta Results: Total reads = 32676008 Total reads for de novo clustering = 24008126 Total reads passing E-value threshold = 25897874 (79.26%) Total reads failing E-value threshold = 6778134 (20.74%) Minimum read length = 76 Maximum read length = 151 Mean read length = 146 By database: /home/jona1883/software/American-Gut/data/AG/BLOOM.fasta 79.26% Total reads passing %id and %coverage thresholds = 1889748 Total OTUs = 10